(1981) Cell substrate contacts illuminated by total internal reflection fluorescence. (1981) The removal of extracellular fibronectin from areas of cell-substrate contact. Plenum Press, New York and London.Īvnur, Z. (1985) Surface and interfacial aspects of biomedical polymers, vol.1 Surface chemistry and physics. This process is experimental and the keywords may be updated as the learning algorithm improves.Īndrade, J.D. These keywords were added by machine and not by the authors. The success of this technique has exceeded our most optimistic predictions.
The theory helped us to understand why IRM images could sometimes be very hard to interpret, and our attention turned to total internal reflection fluorescence as an alternative and superior method of visualizing and measuring cell-to substratum contacts.
Physicseditor alternative for free#
Cell-to-substratum water gaps were indeed detected in certain situations for aldehyde-fixed red blood cells (Gingell & Todd, 1980) and for free living amoebae (Gingell & Vince, 1982b). This led to an interest in interferometry which eventually culminated in a quantitative theory of interference reflection microscopy, IRM, (Gingell & Todd, 1979 Gingell et al., 1982). Since the idea of a long-range force balance implies a gap between the cell membrane and the substratum, it was natural to try to visualize it optically and measure it if possible. In the dark ages, when it was suspected that cell adhesion could be understood solely as a long-range balance of intermolecular attractive and repulsive forces, without direct molecular contact, I joined Dr.V.A.Parsegian to learn how to calculate the size of these forces (Parsegian & Gingell, 1973) and went on to devise several ways of measuring them for cell contacts. A brief explanation of the way in which the topics to be discussed are connected may be helpful. It is my intention to outline the main biophysical studies on cell-to-substratum contact which have been done in this laboratory during the past few years.
0 kommentar(er)
